Microfluidic Bioreactors for Cellular Microarrays

Microfluidic Methods for Protein Microarrays

Protein microarray technology has an enormous potential for in vitro diagnostics (IVD). Miniaturized and parallelized immunoassays are powerful tools to measure dozens of parameters from minute amounts of sample, whilst only requiring small amounts of reagent. Protein microarrays have become well-established research tools in basic and applied research and the first diagnostic products are alre...

Biostickers: Patterned Microfluidic Stickers for Rapid Integration with Microarrays

We present a one-step, reversible, and biocompatible bonding method of a stiff patterned microfluidic "Biosticker", based on off-stoichiometry thiol-ene (OSTE) polymers [1], to state-of-the-art spotted microarray surfaces. The method aims at improving and simplifying the batch back-end processing of microarrays. We illustrate its ease of use in two applications: a high sensitivity flow-through ...

Microfluidic Technologies and Instrumentation for Printing DNA Microarrays

Microfluidic electroporation for cellular analysis and delivery.

Electroporation is a simple yet powerful technique for breaching the cell membrane barrier. The applications of electroporation can be generally divided into two categories: the release of intracellular proteins, nucleic acids and other metabolites for analysis and the delivery of exogenous reagents such as genes, drugs and nanoparticles with therapeutic purposes or for cellular manipulation. I...

Microfluidic Cell Culture Systems for Cellular Analysis

Microfluidic systems have significant implications for the fields of cell biology and cell-based assay as they enable conventional assays to be conducted using an automated and high-throughput approach. Unlike conventional in vitro cell culture methods, microfluidics can provide small and complex structures mimicking the in vivo environment of cells. Recent research has shown that microfluidic ...